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cobalt affinity chromatography  (TaKaRa)


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    Structured Review

    TaKaRa cobalt affinity chromatography
    Cobalt Affinity Chromatography, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cobalt+affinity+chromatography/TALON+Superflow+Metal+Affinity+Resin/pmc12851581-215-5-12
    Average 95 stars, based on 403 article reviews
    cobalt affinity chromatography - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Protease sensor molecules
    Article Snippet: .. Proteins were purified using cobalt affinity chromatography (TALON® Superflow Metal Affinity Resin (Takara Clontech, Australia)) according to the manufacturer's instructions. .. Following elution of the purified protein with 150 mM imidazole, the sample was dialyzed against plasmin cleavage buffer (50 mM Tris (pH 8), 10 mM NaCl and 25 mM lysine) using a cellulose dialysis membrane (12,000 molecular weight cutoff (Sigma)).

    Article Title: Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families
    Article Snippet: .. The NTD containing an 8x-Histidine tag were purified via cobalt affinity chromatography using Talon Metal Affinity Resin (Takara Bio, CA). ..

    Article Title: Identification of genes required for Plasmodium gametocyte-to-sporozoite development in the mosquito vector.
    Article Snippet: .. The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS), pH 7.4. .. Protein samples were analyzed by SDS-PAGE to determine purity prior to their use for immunization in rabbits for the generation of an affinity purified polyclonal antibody (Eurogentec).

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St or the D55C and D59C mutants was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Chromatography:

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St or the D55C and D59C mutants was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Extraction:

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Allosteric effects of the coupling cation in melibiose transporter MelB
    Article Snippet: .. Briefly, MelB St purification by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB
    Article Snippet: Cell growth for the large-scale production of WT MelB St or the D55C and D59C mutants was carried out using the same expression vector and cell strain as used in the functional analyses ( , ). .. Briefly, MelB St purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM. ..

    Article Title: Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Affinity Chromatography:

    Article Title: Protease sensor molecules
    Article Snippet: .. Proteins were purified using cobalt affinity chromatography (TALON® Superflow Metal Affinity Resin (Takara Clontech, Australia)) according to the manufacturer's instructions. .. Following elution of the purified protein with 150 mM imidazole, the sample was dialyzed against plasmin cleavage buffer (50 mM Tris (pH 8), 10 mM NaCl and 25 mM lysine) using a cellulose dialysis membrane (12,000 molecular weight cutoff (Sigma)).

    Article Title: Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families
    Article Snippet: .. The NTD containing an 8x-Histidine tag were purified via cobalt affinity chromatography using Talon Metal Affinity Resin (Takara Bio, CA). ..

    Article Title: Identification of genes required for Plasmodium gametocyte-to-sporozoite development in the mosquito vector.
    Article Snippet: .. The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS), pH 7.4. .. Protein samples were analyzed by SDS-PAGE to determine purity prior to their use for immunization in rabbits for the generation of an affinity purified polyclonal antibody (Eurogentec).

    Saline:

    Article Title: Identification of genes required for Plasmodium gametocyte-to-sporozoite development in the mosquito vector.
    Article Snippet: .. The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS), pH 7.4. .. Protein samples were analyzed by SDS-PAGE to determine purity prior to their use for immunization in rabbits for the generation of an affinity purified polyclonal antibody (Eurogentec).



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